bacteria 507  (ATCC)

Journal: Pharmaceutics

Article Title: Smart-AMPs: Decorated Nanostructured Lipid Carriers for Improved Efficacy of Antimicrobial Peptides in Chronically Infected Burn Wounds

doi: 10.3390/pharmaceutics17081039

Figure Lengend Snippet: Effect of AMP treatment on bioluminescence of A. fischeri over time. ( A ) Absolute bioluminescence levels (in BLU) of untreated and AMP-treated bacteria. ( B ) Relative bioluminescence inhibition (%) in AMP-treated bacteria normalized to the untreated bacteria over time (* p < 0.05; ** p < 0.01).

Article Snippet: The bioluminescent bacteria Aliivibrio fischeri (A. fischeri) strain NRRL-B-11177 was obtained in freeze-dried form from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques: Bacteria, Inhibition

Journal: Pharmaceutics

Article Title: Smart-AMPs: Decorated Nanostructured Lipid Carriers for Improved Efficacy of Antimicrobial Peptides in Chronically Infected Burn Wounds

doi: 10.3390/pharmaceutics17081039

Figure Lengend Snippet: Effects of AMP and AMP-decorated NLC on bioluminescence of A. fischeri in acute infected ( A – C ) and chronically infected wounds ( D – F ) and different wound environments (* p < 0.05; ** p < 0.01; *** p < 0.001).

Article Snippet: The bioluminescent bacteria Aliivibrio fischeri (A. fischeri) strain NRRL-B-11177 was obtained in freeze-dried form from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques: Infection

Journal: Frontiers in Nutrition

Article Title: Bioactive profiles of edible vegetable oils determined using 10D hyphenated comprehensive high-performance thin-layer chromatography (HPTLC×HPTLC) with on-surface metabolism (nanoGIT) and planar bioassays

doi: 10.3389/fnut.2023.1227546

Figure Lengend Snippet: HPTLC RP-18 W chromatograms (A, B) and bioautograms (C, D) of fatty acids ( C8:0 – C18:3 ) and acylglycerols ( MAG , TAG , and DAG ), 10 μg/band each, separated with acetonitrile/water 4:1 ( V / V , with molecular sieve) up to 50 mm, detected (A) at FLD 366 nm after derivatization with rhodamine 6G reagent, and a white light illumination (in remission) after (B) derivatization as reagent sequence first with rhodamine 6G reagent followed by copper sulfate phosphoric acid reagent, (C) Gram-negative Aliivibrio fischeri bioassay (bioluminescence depicted as a greyscale image), and (D) Gram-positive Bacillus subtilis bioassay.

Article Snippet: The luminescent marine Aliivibrio fischeri (DSM 7151) bacteria were purchased from the DSMZ Leibnitz Institut (Berlin, Germany).

Techniques: High Performance Thin Layer Chromatography, Sequencing, Bioassay

Journal: Frontiers in Nutrition

Article Title: Bioactive profiles of edible vegetable oils determined using 10D hyphenated comprehensive high-performance thin-layer chromatography (HPTLC×HPTLC) with on-surface metabolism (nanoGIT) and planar bioassays

doi: 10.3389/fnut.2023.1227546

Figure Lengend Snippet: nanoGIT–HPTLC×HPTLC–bacterial bioassay–FLD/bioluminescence: Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bioautograms showing the antibacterial activity (dark or colorless zones, respectively) of (A, B) digested flaxseed oil and (C) digested coconut oil (10 μg/band each) analyzed as in , detected (A) via the bioluminescence (depicted as a grayscale image) after the A. fischeri bioassay and (B, C) at white light illumination (in remission) after the B. subtilis bioassay; positive controls ( PCs ) were 10–70 μg/band caffeine for A. fischeri bioassay or 10–70 ng/band tetracycline for Bacillus subtilis bioassay.

Article Snippet: The luminescent marine Aliivibrio fischeri (DSM 7151) bacteria were purchased from the DSMZ Leibnitz Institut (Berlin, Germany).

Techniques: Bioassay, Activity Assay